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sirius red fast green collagen staining kit  (Chondrex Inc)


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    Structured Review

    Chondrex Inc sirius red fast green collagen staining kit
    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using <t>Sirius</t> <t>Red/Fast</t> Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
    Sirius Red Fast Green Collagen Staining Kit, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 96/100, based on 296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sirius+red+fast+green+collagen+staining+kit/Sirius+Red%2FFast+Green+Collagen+Staining+Kit/pmc13120883-103-8-14
    Average 96 stars, based on 296 article reviews
    sirius red fast green collagen staining kit - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice"

    Article Title: Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice

    Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

    doi: 10.1093/gerona/glag085

    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
    Figure Legend Snippet: TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.

    Techniques Used: Staining

    Related Articles

    Staining:

    Article Title: Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice.
    Article Snippet: The images were acquired using a ZEISS Axioscan 7 Microscope, and blue-stained areas were quantified using Fiji ImageJ software. .. The semi-quantitative collagen detection kit for total tissue (Sirius Red/Fast Green collagen staining kit, Chondrex, Inc., catalog # 9046, Woodinville, WA) was used to assess the total amount of collagen and non-collagenous proteins in adipose tissue sections from the three experimental groups. ..

    Article Title: Self-assembled nanoparticles of hybrid elastin-like and Oncostatin M polymers for improved wound healing.
    Article Snippet: Oncostatin M (OSM) is a pleiotropic cytokine that can significantly enhance wound healing.. Here, we report on the use of nanoparticles (NPs) formulated from a genetically engineered A200_hOSM protein polymer, which combines an elastin-like recombinamer (A200) with human OSM (hOSM) in the same molecule, aiming at enhancing wound healing processes.. A200_hOSM NPs were obtained by self-assembly and evaluated for their bioactivity in human keratinocytes and fibroblasts.

    Article Title: Influences of ampicillin exposure in early life on the murine gut microbiota and steatotic liver disease associated with western diet.
    Article Snippet: .. The degree of fibrosis (stage 0-4) was evaluated on serial sections, which were stained with Sirius Red–Fast Green Collagen staining kit (Chondrex, Inc., USA, cat.no. ..

    Article Title: IκBζ regulates the development of nonalcoholic fatty liver disease through the attenuation of hepatic steatosis in mice.
    Article Snippet: .. The sections were processed for hematoxylin and eosin staining, Sirius Red staining (Sirius Red/Fast Green Collagen Staining Kit, Chondrex Inc., Woodinville, WA, USA), or immunostaining. .. The frozen sections were subjected to Oil Red O staining (FUJIFILM Wako Pure Chemical, Osaka, Japan).

    Article Title: β-hydroxybutyrate recapitulates the beneficial effects of ketogenic metabolic therapy in polycystic kidney disease
    Article Snippet: Zymo-spin TM IICR column mini-prep , Zymo Research , Cat# D4068. .. Sirius Red/Fast Green collagen staining kit , Chondrex , Cat# 9046. .. IGF-1 Rat ELISA Kit , Invitrogen , Cat# ERIGF1.

    Article Title: Microengineered Multicomponent Hydrogel Fibers: Combining Polyelectrolyte Complexation and Microfluidics.
    Article Snippet: Subscriber access provided by CORNELL UNIVERSITY LIBRARY ACS Biomaterials Science & Engineering is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

    Article Title: Effect of Thyroxine on Transforming Growth Factor β1, Collagen I, and V Expression in Keratoconus Corneal Fibroblasts and Keratocytes, in Vitro.
    Article Snippet: Background: Keratoconus (KC) is a corneal disorder, associated with oxidative stress, hypoxia and as several times discussed, potentially with thyroid gland dysfunction.. We aimed to investigate the effect of thyroxine on transforming growth factor β1 (TGF-β1), collagen I and V (Col I and V) expression in human corneal fibroblasts (HCFs) and human keratocytes of KC corneas, in vitro.. Methods: Primary human KC-keratocytes and normal keratocytes were isolated and cultured as corneal fibroblasts or keratocytes.

    Article Title: Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice
    Article Snippet: The images were acquired using a ZEISS Axioscan 7 Microscope, and blue-stained areas were quantified using Fiji ImageJ software. .. The semiquantitative collagen detection kit for total tissue (Sirius Red/Fast Green collagen staining kit, Chondrex, Inc., catalog # 9046, Woodinville, WA) was used to assess the total amount of collagen and noncollagenous proteins in adipose tissue sections from the three experimental groups. ..

    Immunostaining:

    Article Title: IκBζ regulates the development of nonalcoholic fatty liver disease through the attenuation of hepatic steatosis in mice.
    Article Snippet: .. The sections were processed for hematoxylin and eosin staining, Sirius Red staining (Sirius Red/Fast Green Collagen Staining Kit, Chondrex Inc., Woodinville, WA, USA), or immunostaining. .. The frozen sections were subjected to Oil Red O staining (FUJIFILM Wako Pure Chemical, Osaka, Japan).

    Colorimetric Assay:

    Article Title: Microengineered Multicomponent Hydrogel Fibers: Combining Polyelectrolyte Complexation and Microfluidics.
    Article Snippet: Subscriber access provided by CORNELL UNIVERSITY LIBRARY ACS Biomaterials Science & Engineering is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.



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    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using <t>Sirius</t> <t>Red/Fast</t> Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
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    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using <t>Sirius</t> <t>Red/Fast</t> Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
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    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using <t>Sirius</t> <t>Red/Fast</t> Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
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    Image Search Results


    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.

    Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

    Article Title: Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice

    doi: 10.1093/gerona/glag085

    Figure Lengend Snippet: TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.

    Article Snippet: The semiquantitative collagen detection kit for total tissue (Sirius Red/Fast Green collagen staining kit, Chondrex, Inc., catalog # 9046, Woodinville, WA) was used to assess the total amount of collagen and noncollagenous proteins in adipose tissue sections from the three experimental groups.

    Techniques: Staining